Vaccine I Cell Therapy Equipments

Product Details:

CelCradle™ is a disposable bioreactor capable of high density cell culture for protein expression, virus and monoclonal antibody production. It is designed based on the concept of bellow-induced intermittent flow of media and air through porous matrices, where cells reside. This provides a low shear, high aeration, and foam-free culture environment.

During operation, the CelCradle™ bottle is partially filled with media and inoculated with cells. The media is raised and lowered alternately to submerge and expose the matrices, creating a dynamic interface between air and media on cell surface to maximize nutrient uptake and oxygen transfer.

CelCradle™ is the only single-use packed bed bioreactor that has linear scalability from bench-scale to production scale with automated cell harvesting.

Features

• Fully Stainless Steel – SS304L
• Pre-sterilized, ready-to-use, disposable
• Low shear stress, foam-free, no O₂ limitation
• Large surface area: pne CelCradle™ bottle has a specific surface area of 15,000 cm², comparable to 20 – 30 roller bottles.
• Compact design: fits in 6ft³ CO₂ incubator
• Controlled by Esco Sentinel Gold
• Capacity to produce hundreds of milligrams to several grams of secreted proteins or monoclonal antibodies per month in four bottles
• Few parameters for easy optimization
• Choice of batch, fed-batch, or perfusion culture modes
• Scalability by directly multiplying number of bottles used
• Compatible with most serum-free media
• Specially treated micro carrier surface to grow most anchorage-dependent cells and allow easy harvest of whole cells, cell components or secreted proteins

Applications

• Mammalian and insect cell culture
• Protein and virus production
• Monoclonal antibody production
• Proteome research
• Drug discovery
• Pharmacokinetics study
• Gene and cell therapy

 

Product Details:

TideCell® High Density bioreactor system is the extended large scale system of the lab scale CelCradle® bioreactor system. In this system, a matrix vessel is packed with porous BioNoc™ II carriers and functions as an artificial lung with huge contact surface for nutrition and aeration. Culture medium is periodically drawn from an external medium bag (mixing bag) into the matrix vessel to submerge the carriers and withdrawn from the vessel to expose the carriers. As a result, cells entrapped within the carriers are able to receive sufficient nutrient and oxygen, while metabolic wastes such as lactate and carbon dioxide can be efficiently carried away in a gentle manner.

Features

• Compact and high cell density (up to 6x10⁷ cells/ml)
• Foam-free, low shear stress, high oxygen transfer
• Flexible system: combination of matrix vessels from 2L to 100L and mixing bags from 20L to 1000L
• Single-use, autoclavable, or hybrid system
• Linear scalability
• Sampling ports for matrices in closed system thus avoiding contamination and lowering operator risks

Applications

• Mammalian and insect cell culture
• Protein and virus production
• Monoclonal antibody production
• Gene and cell therapy products manufacturing

Tide motion pertains to the oscillation of culture medium into and out of the matrix vessel that intermittently exposes the cells to aeration and nutrition.

The VacciXcell™ Hybrid Reactor is a laboratory scale bioreactor system that supports both the growth of suspension and adherent cells. It is capable of processing adherent cell culture using macrocarriers or microcarriers, suspension culture and fermentation.